UMP

Endothelium

Endothelium in cardiology

Vascular endothelial cells are tested in the context of acute coronary syndromes (ACS) and related percutaneous coronary intervention (PCI) with stent implantation and applied pharmacotherapy. PCI opens clogged coronary arteries but, at the same time, damages the endothelial cell layer leading to pathological vessel remodeling called restenosis. Experiments were conducted in in vitro culture conditions, partially mimicking the in vivo environment. Additionally, sera taken from patients treated with PCI at different time points were also analyzed.

Selected publications

  1. Pyda M., Korybalska K., Książek K., Grajek S., Łanocha M., Lesiak M., Wiśniewska-Elnur J., Olasińska A., Bręborowicz A., Cieśliński A., Witowski J. Heparin modulates blood VEGF levels in patients undergoing primary percutaneous coronary intervention by promoting VEGF binding to endothelial cells. Am J Cardiol. 2006; 98: 902-905.
    https://doi.org/10.1016/j.amjcard.2006.04.028
  2. Korybalska K., Pyda M., Grajek S., Łanocha M., Bręborowicz A., Witowski J.Serum profiles of monocyte chemoattractant protein-1 as a biomarker for patient recovering from myocardial infarction. Clin Res Cardiol. 2010; 99: 315-322.
    https://doi.org/10.1007/s00392-010-0122-1
  3. Korybalska K., Pyda M., Kawka E., Grajek S., Bręborowicz A., Witowski J. Interpretation of elevated serum VEGF concentrations in patients with myocardial infarction. Cytokines 2011; 54: 74-78.
    https://doi.org/10.1016/j.cyto.2011.01.003
  4. Pyda M., Korybalska K., Grajek S., Książek K., Bręborowicz A., Witowski J. Endothelial cell growth response to stimulation with serum from patients with acute ST-elevation myocardial infarction. Int J Cardiol. 2012; 159: 235-237.
    https://doi.org/10.1016/j.ijcard.2012.05.103
  5. Korybalska K., Kawka E., Bręborowicz A., Witowski J. Atorvastatin does not impair endothelial cell wound healing in an in vitro model of vascular injury. J Physiol Pharmacol. 2012; 63: 389-95.
    http://www.jpp.krakow.pl/
  6. Korybalska K., Kawka E., Kusch A., Aregger F., Dragun D., Jörres A., Bręborowicz , Witowski J. Recovery of senescent endothelial cells from injury. J Gerontol A Biol Sci Med Sci. 2013; 68: 250-257.
    https://doi.org/10.1093/gerona/gls169
  7. Korybalska K.,Kawka E., Bręborowicz A., Witowski J. The role of mTOR inhibitors and HMG-CoA reductase inhibitors on young and old endothelial cells functions, critical for re-endothelization after percutaneous coronary intervention (PCI): an in vitro J Physiol Pharmacol 2017; 68: 397-405.
    http://www.jpp.krakow.pl/
  8. Korybalska K., Rutkowski R., Łuczak J., Czepulis N., Karpiński K., Witowski J. The role of P2Y12 receptor blockers on angiogenic properties of endothelial cells: an in vitro study J Physiol Pharmacol, 2018; 69: 547-560.
    https://doi.org/10.26402/jpp.2018.4.06
  9. Catar R., Moll G., Hosp I., Simon M., Luecht Ch., Zhao H., Wu D., Chen L., Kamhieh-Milz J., Korybalska K., Zickler D., Witowski J. Transcriptional regulation of thrombin-induced endothelial VEGF induction and proangiogenic response. Cells 2021;10: 910 [s. 1-14].
    https://doi.org/10.3390/cells10040910
  10. Sikorska D., Kamińska D, Catar R, Wu D, Zhao H, Wang P, Kamhieh-Milz J, Banasik M, Kusztal M, Cielecka M, Zakliczyński M, Rutkowski R, Korybalska K, Heidecke H, Moll G, Samborski W. Nailfold videocapillaroscopy for non-invasive assessment of microcirculation and prognostic correlation with endothelial dysfunction, cardiovascular risk factors, and non-HLA antibodies in heart transplant recipients: a pilot study. J. Clin. Med. 2023; 12: art. 2302  https://doi.org/10.3390/jcm12062302
  11. Antoniak K, Studzińska-Sroka E,  Szymański M, Dudek-Makuch M, Cielecka-Piontek J, and Korybalska K. Antiangiogenic anti-inflammatory and antioxidant properties of Bidens tripartite herb, Galium verum herb and Rumex hydrolapathum root. Molecules, 2023; 28: 4966. https://doi.org/10.3390/molecules28134966
  12. Kamhieh-Milz J, Chen L, Goettsch C, Pfefferkorn AM, Hofmann A, Brunssen C, Müller G, Walther T, Ashraf M, Moll G, Morawietz H, Witowski J, Catar R. Angiotensin II promotes endothelin 1 production by regulating NOX2 activity through transcription factor Oct-1. Arterioscler Thromb Vasc Biol 2023 (in press).
    doi: 10.1161/ATVBAHA.122.318764

 

Endothelium in obesity

Studies focus on people with abnormal body weight treated with caloric restriction, supplementation, or fasting. The most attention was paid to improving vascular endothelial cells, metabolic and organ-specific functions.

Selected publications:

  1. Korybalska K, Swora-Cwynar E, Łuczak J, Kanikowska A, Czepulis N, Rutkowski R, Bręborowicz A, Grzymisławski M, Witowski J. Association of endothelial proliferation with the magnitude of weight loss during calorie restriction. Angiogenesis 2016; 19:407-419.
    https://doi.org/10.1007/s10456-016-9516-7
  2. Korybalska K., Łuczak J., Swora-Cwynar E., Kanikowska A., Czepulis N., Kanikowska D., Skalisz H., Bręborowicz A., Grzymisławski M., Witowski J. Weight loss – dependent and independent effects of moderate calorie restriction on endothelial cell function in obesity. J Physiol Pharmacol 2017: 68: 597-608.
    http://www.jpp.krakow.pl/
  3. Korybalska K. Angiogenesis in adipose tissue. How can caloric restriction affect obesity-related endothelial dysfunction. 2018; str: 251-286 (rozdział 11); Książka „Endothelial dysfunction” pod redakcją dr Helenny Lenasi, wydawnictwo InTech.
    https://www.intechopen.com/chapters/58606
  4. Kanikowska D., Korybalska K., Mickiewicz A., Rutkowski R., Kuchta A., Sato M.,, Kreft E.,, Fijałkowski M., , Gruchała M.,, Jankowski M.,, Bręborowicz A., Witowski J. Flaxseed (Linum Usitatissimum) supplementation in patients undergoing lipoprotein apheresis for severe hyperlipidemia - a pilot study. Nutrients 2020;12: art. 1137 [s. 1-10].
    https://doi.org/10.3390/nu12041137
  5. Budzanowski J., Hiczkiewicz J., Łojewska K., Kawka E., Rutkowski R., Korybalska K., Predictors of early-recurrance atrial fibrillation after catheter ablation in women and men with abnormal body weight. Clin. Med. 2021;10: art. 2694 [s. 1-15].
    https://doi.org/10.3390/jcm10122694
  6. Kanikowska D., Kanikowska A., Swora-Cwynar E., Grzymisławski M., Sato M., Bręborowicz A., Witowski J., Korybalska K. Moderate caloric restriction partially improved oxidative stress markers in obese humans. Antioxidants (Basel) 2021;10: 1018 [s. 1-14].
    https://doi.org/10.3390/antiox10071018
  7. Kanikowska D., Malińska A.,Mickiewicz A., Zawada A.,Rutkowski R., Pawlaczyk K., Sato M., Bręborowicz A., Witowski J., Korybalska K. Effect of flaxseed ( Linum usitatissimum) supplementation on vascular endothelial cell morphology and function in patients with dyslipidaemia-A preliminary observation. Nutrients 2022;14: art. 2879 [s.1-17].
    https://doi.org/10.3390/nu14142879
  8. Kanikowska D, Kanikowska A, Swora-Cwynar E, Grzymisławski M, Sato M, Breborowicz A, Witowski J, Korybalska K. Soluble receptor for advanced glycation end products (sRAGE) correlates with obesity-related parameters, and it is not easy to be modified by moderate caloric restriction in obese humans. J Physiol Pharmacol. 2022 Aug;73(4). doi: 10.26402/jpp.2022.4.06.
    https://doi.org/10.26402/jpp.2022.4.06

 

Endothelium – uremia and renal replacement therapy

The research concerns the impact of uremia and dialysis on endothelial cells from various parts of the vascular system. Different changes in the function of the venous and arterial endothelium in the conditions of uremia have been demonstrated. The process of hemodialysis and peritoneal dialysis modifies the properties of vascular endothelial cells. Factors that may reduce unfavorable changes in endothelial cells in these conditions have been indicated.

Selected publications

  1. Oleśkowska-Florek W, Połubinska A, Baum E, Matecka M, Pyda M, Pawlaczyk K, Bręborowicz A. Hemodialysis-induced changes in the blood composition affect function of the endothelium. Hemodial Int. 2014 ;18:650-656.
    doi: 10.1111/hdi.12148
  2. .Baum E, Maćkowiak B, Sosińska-Zawierucha P, Pawlaczyk K, Kołodziejczak B, Breborowicz A. Higher Serum Hepatocyte Growth Factor Concentration is Associated with Better Preservation of GFR in Hemodialysis Patients. Kidney Blood Press Res. 2017;42:1175-1182.
    doi: 10.1159/000485864
  3. Sosinska-Zawierucha P, Mackowiak B, Breborowicz A. N-Acetylcysteine and Sulodexide Reduce the Prothrombotic Effect of Uremic Serum on the Venous Endothelial Cells. Kidney Blood Press Res. 2019;44:277-285
    doi: 10.1159/000499879
  4. Sosinska-Zawierucha P, Baum E, Mackowiak B, Misian M, Bręborowicz A. Different Effect of Hemodialysis on Function of Human Arterial and Venous Endothelial Cells. Blood Purif. 2019;47:346-350.
    doi: 10.1159/000495342
  5. Sosińska-Zawierucha P, Bręborowicz A. Uremic serum induces prothrombotic changes in venous endothelial cells and inflammatory changes in aortic endothelial cells. Ren Fail. 2021;43:401-405.
    doi: 10.1080/0886022X.2021.1890617
  6. Misian M, Baum E, Bręborowicz A. Secretory activity of the coronary artery endothelial cells in conditions of the peritoneal dialysis. Ren Fail. 2022;44: 54-61.
    doi: 10.1080/0886022X.2021.2023024.

 

Endothelium – peripheral blood vessels

The research was carried out on arterial and venous endothelial cells. The effect on the function of these cells was assessed by hyperglycemia, sera from patients with lower extremity venous disease or peripheral arterial disease. Factors that reduce the adverse effects of these factors on endothelial cells are indicated.

Selected publications

  1. Połubińska A, Staniszewski R, Baum E, Sumińska-Jasińska K, Bręborowicz A. Sulodexide modifies intravascular homeostasis what affects function of the endothelium. Adv Med Sci. 2013;58:304-10.
    doi: 10.2478/ams-2013-0016
  2. Ciszewicz M, Polubinska A, Antoniewicz A, Suminska-Jasinska K, Breborowicz A. Sulodexide suppresses inflammation in human endothelial cells and prevents glucose cytotoxicity. Transl Res. 2009 ;153:118-123.
    doi: 10.1016/j.trsl.2008.12.007.
  3. Sosińska P, Baum E, Maćkowiak B, Maj M, Sumińska-Jasińska K, Staniszewski R, Bręborowicz A. Sulodexide Reduces the Proinflammatory Effect of Serum from Patients with Peripheral Artery Disease in Human Arterial Endothelial Cells. Cell Physiol Biochem. 2016;40:1005-1012.
    doi: 10.1159/000453157
  4. Gericke A, Suminska-Jasińska K, Bręborowicz A. Sulodexide reduces glucose induced senescence in human retinal endothelial cells. Sci Rep. 2021 Jun 1;11(1):11532.
    doi: 10.1038/s41598-021-90987-w.
  5. Dauth A, Bręborowicz A, Ruan Y, Tang Q, Zadeh JK, Böhm EW, Pfeiffer N, Khedkar PH, Patzak A, Vujacic-Mirski K, Daiber A, Gericke A. Sulodexide Prevents Hyperglycemia-Induced Endothelial Dysfunction and Oxidative Stress in Porcine Retinal Arterioles. Antioxidants (Basel). 2023 ;12:388.
    doi: 10.3390/antiox12020388

 

Date of update: 6 july 2023 by Administrator